Up-Regulation of Differentiation Related Gene-1 Expression in the Androgen Independent Prostate Cancer / 대한비뇨기과학회지
Korean Journal of Urology
; : 637-642, 2003.
Article
de Ko
| WPRIM
| ID: wpr-174532
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: To determine whether the up-regulation of the differentiation related gene (Drg-1) enhances the progression of prostate cancer into the androgen independent phenotype, via escaping androgen signal transduction. MATERIALS AND METHODS: A full length of Drg-1 cDNA was obtained using the Drg-1 primer, which was inserted into LNCaP cells. The sensitivities of dihydrotestosterone and bicalutamide were then examined. In addition, the level of the Drg-1 gene expression was examined in derivatives of the LNCaP cell lines obtained from the orthotopic animal model. RESULTS: The Drg-1 transfected LNCaP cells, which highly expressed the Drg-1, were established (LNCaP/D2). The LNCaP/D2 slowly grew in a culture medium, supplemented with 10% charcoal stripped fetal bovine serum, whereas the control cells did not. When the sensitivities of DHT and bicalutamide were examined, the PC-3 and LNCaP/D2 cells were not sensitive to either. The metastatic androgen independent prostate cancer cells (LNCaP-AI-Lung), which were obtained from the orthotopic animal model, showed higher levels of Drg-1 expression than the parental cells. CONCLUSIONS: Androgen dependent prostate cancer cells, expressing high levels of the Drg-1 gene, behave like androgen independent prostate cancer cells. This finding suggest that the Drg-1 gene may play an important role in the initiation of an androgen-independent state.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Parents
/
Phénotype
/
Prostate
/
Tumeurs de la prostate
/
5alpha-Dihydrotestostérone
/
Nations Unies
/
Transduction du signal
/
Expression des gènes
/
Régulation positive
/
Lignée cellulaire
Type d'étude:
Prognostic_studies
Limites du sujet:
Humans
langue:
Ko
Texte intégral:
Korean Journal of Urology
Année:
2003
Type:
Article