Screening of Epidermal Growth Factor Receptor Gene Mutation in Non-Small Cell Lung Cancer Using a PCR-Based Enzymatic Digestion Method
Journal of Lung Cancer
; : 77-83, 2012.
Article
de En
| WPRIM
| ID: wpr-178022
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: We applied a simplified method using polymerase chain reaction (PCR)-based enzymatic digestion for the detection of epidermal growth factor receptor (EGFR) mutation. MATERIALS AND METHODS: We selected 74 samples of adenocarcinoma of the lung with EGFR exons 19 and 21 that had been previously sequenced. We designed PCR primers and chose a DNA restriction enzyme. Seventy four additional lung cancer samples were tested as a test set. For test sets, the PCR-based method was performed first, followed by validation of the result by DNA sequencing. RESULTS: In the first sample group, we found 15 (20.3%) mutations in exon 19, and 9 (12.2%) mutations in exon 21 using the sequencing method. By using the PCR-based method, we were able to identify all of the mutated samples detected by the sequencing method. The PCR-based method also detected mutations in exon 19 in three additional samples and in exon 21 in one additional sample. In the second sample group, by performing the PCR-based method, we found 10 (13.5%) and 7 (9.5%) mutations in exons 19 and 21, respectively. Additional mutations in exon 19 were identified in 2 samples by the sequencing method. However, the sequencing method failed to identify a mutation in exon 21 in one sample. CONCLUSION: The sensitivity of the PCR-based enzymatic digestion method seems to be comparable to that of the traditional sequencing method for detecting EGFR mutations. Our method can be widely used as a screening test to select patients who may benefit from EGFR targeted therapy.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
ADN
/
Adénocarcinome
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Cartographie de restriction
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DNA restriction enzymes
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Dépistage de masse
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Réaction de polymérisation en chaîne
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Exons
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Carcinome pulmonaire non à petites cellules
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Gènes erbB-1
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Digestion
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limites du sujet:
Humans
langue:
En
Texte intégral:
Journal of Lung Cancer
Année:
2012
Type:
Article