Contralateral allodynia and central change in the chronic post-ischemic pain model rats / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 419-424, 2009.
Article
Dans Coréen
| WPRIM
| ID: wpr-179767
ABSTRACT
BACKGROUND:
Mirror-image allodynia is a mysterious phenomenon that occurs in association with many clinical pain syndromes including complex regional pain syndromes (CRPS). Underlying mechanisms for the development of such pain are still a matter of investigation. Several studies suggest that activation of the N-methyl-D-aspartate (NMDA) receptor is essential for central sensitization as a base for persistent pain. The aim is to assess whether alteration of NMDA receptor expression correlates with the contralateral allodynia in the chronic post-ischemia pain (CPIP) model rats representing CRPS-Type I.METHODS:
Application of a tight-fitting tourniquet for a period of 3 hours before reperfusion produced CPIP in male Sprague-Dawley rats. The mechanical paw withdrawal thresholds to von Frey stimuli (using a dynamic plantar aesthesiometer) were measured as pain indicators in ipsilateral and contralateral hindpaws. Phosphorylation of the NMDA receptor 1 subunit (pNR1), assessed with Western blot, was measured in the contralateral L4-6 spinal cord.RESULTS:
Ipsilateral and contralateral mechanical allodynia is present at 4 hours after reperfusion, peaked at 3 days, and continued for 7 days after reperfusion. The relative density of pNR1 of CPIP rats significantly decreased in the contralateral L4-6 spinal cord compared to baseline value (P < 0.05). There was significant correlation between paw withdrawal threshold and the relative density of pNR1 (ipsilateral; R2 = 0.75, P < 0.01, contralateral; R2 = 0.60, P < 0.01).CONCLUSIONS:
These data suggest that pNR1 is correlated to the contralateral mechanical allodynia in CPIP rats.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphorylation
/
Prostaglandines E
/
Gravité spécifique
/
Moelle spinale
/
Garrots
/
Reperfusion
/
Technique de Western
/
N-Méthyl-aspartate
/
Rat Sprague-Dawley
/
Syndrome douloureux régional complexe
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Coréen
Texte intégral:
Korean Journal of Anesthesiology
Année:
2009
Type:
Article
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