Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 496-501, 2013.
Article
Dans Coréen
| WPRIM
| ID: wpr-181312
ABSTRACT
PURPOSE:
To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC).METHODS:
Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20, and 50 microm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay.RESULTS:
DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS (p < 0.05) and improved cellular activity significantly after treatment with t-BHP (p < 0.05). DPD did not affect the generation of nitric oxides.CONCLUSIONS:
DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oxazines
/
Réseau trabéculaire de la sclère
/
Xanthènes
/
Espèces réactives de l'oxygène
/
Stress oxydatif
/
2-Hydroperoxy-2-méthyl-propane
/
Dipyridamole
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Journal of the Korean Ophthalmological Society
Année:
2013
Type:
Article
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