The Effect of Tissue Plasminogen Activator on TGF-beta1 Pre-Treated Human Mesothelial Cell Line / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
; : 405-415, 2011.
Article
de Ko
| WPRIM
| ID: wpr-181454
Bibliothèque responsable:
WPRO
ABSTRACT
BACKGROUND: In an effort to find alternative therapeutic agents to prevent excessive fibrosis as a sequela to complicated parapneumonic effusion or empyema, we examined the effect of tissue plasminogen activator (tPA) as a fibrinolytic agent combined with talc or transforming growth factor (TGF)-beta1 in a human pleural mesothelial cell line, MeT-5A. METHODS: MeT-5A cells were stimulated with various doses of talc, doxycycline or TGF-beta1 for 24 h and then were treated with tPA for an additional 24 h. Cell viability was measured by MTT assay. The production of interleukin (IL)-8 and vascular endothelial growth factor (VEGF) in the culture supernatants was measured by ELISA. Real-time PCR was carried out for measurement of type I collagen mRNA. RESULTS: MeT-5A cells treated with talc showed a dose-dependent increase in production of IL-8. Talc also increased production of type I collagen mRNA at low doses, but talc did not influence the induction of VEGF. Addition of tPA to talc-stimulated cells showed further increases in the production of IL-8, but tPA did not influence the production of VEGF or type I collagen mRNA. TGF-beta1 increased the production of both VEGF and collagen type I mRNA, both of which were effectively inhibited by additional tPA treatment in MeT-5A cells. CONCLUSION: TGF-beta1 is a potent inducer of collagen synthesis without induction of IL-8 in MeT-5A cells. Addition of tPA after TGF-beta1 stimulation inhibited further fibrosis by direct inhibition of collagen mRNA synthesis as well as by inhibition of VEGF production.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Talc
/
Fibrose
/
ARN messager
/
Test ELISA
/
Facteurs de croissance transformants
/
Lignée cellulaire
/
Survie cellulaire
/
Interleukine-8
/
Collagène
/
Interleukines
Limites du sujet:
Humans
langue:
Ko
Texte intégral:
Tuberculosis and Respiratory Diseases
Année:
2011
Type:
Article