The Effects of Glutathione on Human Cancer Cells before and after the Application of Cisplatin / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 161-165, 2004.
Article
Dans Coréen
| WPRIM
| ID: wpr-182588
ABSTRACT
OBJECTIVE:
The purpose of this study is to determine the time-dependant effects of Glutathione on the Cisplatin-induced cytotoxicity of human cervical carcinoma cells.METHODS:
Two human cervical carcinoma cells, SiHa (squamous cell carcinoma cell), and CaSki (epidermoid metastatic carcinoma cell) were cultivated with RPMI1640 media. Reduced glutathione (GSH) and 2-oxo 4-thiazolidine carboxylic acid (OTC) were added one hour before and after Cisplatin (2-50 micro M/ml) was applied. The cells were incubated an additional 24 hours and viable cells were examined using a 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay.RESULTS:
The MTT reduction rate of cisplatin-treated cervical carcinoma cells was increased significantly by the addition of glutathione (5 mM) or OTC (5 mM) both one hour before and after Cisplatin. A difference between MTT reduction rates one hour before and after cisplatin were not observed in either GSH or OTC.CONCLUSION:
These results suggest that GSH and OTC have a protective effect on cisplatin-induced toxicity, and that this effect is about the same whether the agents were applied before or after the Cisplatin.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cisplatine
/
Glutathion
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Korean Journal of Obstetrics and Gynecology
Année:
2004
Type:
Article
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