Antioxidant Effect of Captopril and Enalapril on Reactive Oxygen Species-Induced Endothelial Dysfunction in the Rabbit Abdominal Aorta
The Korean Journal of Thoracic and Cardiovascular Surgery
; : 14-21, 2013.
Article
de En
| WPRIM
| ID: wpr-184565
Bibliothèque responsable:
WPRO
ABSTRACT
BACKGROUND: Reactive oxygen species (ROS) are known to be related to cardiovascular diseases. Many studies have demonstrated that angiotensin-converting enzyme inhibitors have beneficial effects against ROS. We investigated the antioxidant effect of captopril and enalapril in nitric oxide mediated vascular endothelium-dependent relaxations. MATERIALS AND METHODS: Isolated rabbit abdominal aorta ring segments were exposed to ROS by electrolysis of the organ bath medium (Krebs-Henseleit solution) after pretreatment with various concentrations (range, 10-5 to 3x10-4 M) of captopril and enalapril. Before and after electrolysis, the endothelial function was measured by preconstricting the vessels with norepinephrine (10-6 M) followed by the cumulative addition of acetylcholine (range, 3x10-8 to 10-6 M). The relevance of the superoxide anion and hydrogen peroxide scavenging effect of captopril and enalapril was investigated using additional pretreatments of diethyldithiocarbamate (DETCA, 0.5 mM), an inhibitor of Cu/Zn superoxide dismutase, and 3-amino-1,2,4-triazole (3AT, 50 mM), an inhibitor of catalase. RESULTS: Both captopril and enalapril preserved vascular endothelium-dependent relaxation after exposure to ROS in a dose-dependent manner (p<0.0001). Pretreatment with DETCA attenuated the antioxidant effect of captopril and enalapril (p<0.0001), but pretreatment with 3AT did not have an effect. CONCLUSION: Both captopril and enalapril protect endothelium against ROS in a dose-dependent fashion in isolated rabbit abdominal aortas. This protective effect is related to superoxide anion scavenging.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Aorte abdominale
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Oxygène
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Superoxide dismutase
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Vasodilatation
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Bains
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Inhibiteurs de l'enzyme de conversion de l'angiotensine
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Captopril
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Énalapril
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Maladies cardiovasculaires
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Acétylcholine
langue:
En
Texte intégral:
The Korean Journal of Thoracic and Cardiovascular Surgery
Année:
2013
Type:
Article