Efficacy of AlloDerm(R) and Chitosan for Generating Cartilage in Free Perichondrial Graft

ABSTRACT

Many qualitative studies examining the chondrogenic potential of perichondrium have suggested that it may be useful for correction of microtia or for reconstruction of trachea, nasal septum, ala and eyelids, as well as to resurface the temporomandibular joint and small joints in the hand. This study was designed to evaluate cartilage formation by free rabbit auricular perichondrium placed on the muscle fascia of the back of the rabbit for 8 weeks. In addition to free perichondrial graft, AlloDerm(R) and Chitosan, as a scaffold wrapped with perichondrium, were transplanted to the same site respectively. In each case, the chondrogenic potential of perichondrium was examined. The new cartilage was morphologically indistinguishable from normal cartilage. Histologic differences were observed and measured under the light microscope. The mean cartilage thickness for free perichondrium was 0.38 +/- 0.01 mm, 0.64 +/- 0.04 mm for AlloDerm(R) , and 0.55 +/- 0.03 mm for Chitosan after 8 weeks. AlloDerm(R) and Chitosan contributed to produce significantly more neocartilage formation compared with perichondrial graft alone(p < 0.05). No statistic significance was found between AlloDerm(R) and Chitosan grafts. The above result shows AlloDerm(R) and Chitosan could act as a scaffold for generating cartilage and promote the effect on the chondrogenesis of perichondrium.