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Defects in the differentiation and function of bone marrow-derived dendritic cells in non-obese diabetic mice
Journal of Korean Medical Science ; : 217-223, 2000.
Article Dans Anglais | WPRIM | ID: wpr-18567
ABSTRACT
Due to their high immunostimulatory ability as well as the critical role they play in the maintenance of self-tolerance, dendritic cells have been implicated in the pathogenesis of autoimmune diseases. The non-obese diabetic (NOD) mouse is an animal model of autoimmune type 1 diabetes, in which pancreatic beta cells are selectively destroyed mainly by T cell-mediated immune responses. To elucidate initiation mechanisms of beta cell-specific autoimmunity, we attempted to generate bone marrow-derived dendritic cells from NOD mice. However, our results showed low proliferative response of NOD bone marrow cells and some defects in the differentiation into the myeloid dendritic cells. NOD dendritic cells showed lower expressions of MHC class II, B7-1, B7-2 and CD40, compared with C57BL/6 dendritic cells. In mixed lymphocyte reactions, stimulatory activities of NOD dendritic cells were also weak. Treatment with LPS, INF-gamma and anti-CD40 stimulated NOD dendritic cells to produce IL-12p70. The amount of IL-12, however, appeared to be lower than that of C57BL/6. Results of the present study indicated that there may be some defects in the development of NOD dendritic cells in the bone marrow, which might have an impact on the breakdown of self tolerance.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Maladies auto-immunes / Cellules dendritiques / Cellules de la moelle osseuse / Test ELISA / Différenciation cellulaire / Lipopolysaccharides / Facteur de stimulation des colonies de granulocytes et de macrophages / Interleukine-4 / Souris de lignée NOD / Interleukine-12 Type d'étude: Étude pronostique Limites du sujet: Animaux langue: Anglais Texte intégral: Journal of Korean Medical Science Année: 2000 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Maladies auto-immunes / Cellules dendritiques / Cellules de la moelle osseuse / Test ELISA / Différenciation cellulaire / Lipopolysaccharides / Facteur de stimulation des colonies de granulocytes et de macrophages / Interleukine-4 / Souris de lignée NOD / Interleukine-12 Type d'étude: Étude pronostique Limites du sujet: Animaux langue: Anglais Texte intégral: Journal of Korean Medical Science Année: 2000 Type: Article