Combined Application of Bicuculline and 4-Aminopyridine-Induced Epileptiform Activity are Resistant to Conventional Anticonvulsants and AMPA in Young Rat Visual Cortex / 대한간질학회지
Journal of Korean Epilepsy Society
;
: 35-40, 2006.
Article
Dans Coréen
| WPRIM
| ID: wpr-187236
ABSTRACT
PURPOSE:
The goal of the present study was to find out whether blockade of GABAergic synaptic transmission by bicuculline (BIC) in the presence of 4-aminopyridine (4-AP) would lead to expression or suppression of epileptiform activity in the immature brain and to observe the effect of commonly used anticonvulsants (valproic acid (VPA), carbamazepine (CBZP)) and 6-cyano-7-nitroquinoxalinedione (CNQX) on that epileptiform activity.METHODS:
The visual cortex slices were obtained from 14-18 day-old Sprague-Dawley rats. Extracellular cellular recording was performed to observe the induction of epileptiform activity perfused by artificial CSF (ACSF) with combined application of BIC and 4-AP and the effect of VPA, CBZP and CNQX on that epileptiform activity for at least 1 hour.RESULTS:
Epileptiform activity perfused by ACSF with combined application of BIC and 4-AP was insensitive to commonly used anticonvulsants (VPA, CBZP) and sensitive to CNQX.CONCLUSION:
This study suggests that the epileptiform activity induced by combined application of BIC and 4-AP is present even in immature visual cortex slices. And, the blockade of GABAergic inhibition by BIC under 4-AP showed the increase of immature brain excitability as mature brain. The attenuation of that epileptiform activity by a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) antagonist (CNQX) showed that AMPA receptor had relevance to the induction of that epileptiform activity.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Crises épileptiques
/
Cortex visuel
/
Bicuculline
/
Encéphale
/
Carbamazépine
/
4-Amino-pyridine
/
Rat Sprague-Dawley
/
Récepteur de l'AMPA
/
AMPA
/
Transmission synaptique
Limites du sujet:
Animaux
langue:
Coréen
Texte intégral:
Journal of Korean Epilepsy Society
Année:
2006
Type:
Article
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