Silencing IL-23 expression by a small hairpin RNA protects against asthma in mice
Experimental & Molecular Medicine
; : 197-204, 2011.
Article
de En
| WPRIM
| ID: wpr-187633
Bibliothèque responsable:
WPRO
ABSTRACT
To determine the impact of IL-23 knockdown by RNA interference on the development and severity of ovalbumin (OVA)-induced asthmatic inflammation, and the potential mechanisms in mice, the IL-23-specific RNAi-expressing pSRZsi-IL-23p19 plasmid was constructed and inhaled into OVA-sensitized mice before each challenge, as compared with that of control mice treated with alum or budesonide. Inhalation of the pSRZsi-IL-23p19, significantly reduced the levels of OVA-challenge induced IL-23 in the lung tissues by nearly 75%, determined by RT-PCR. In addition, knockdown of IL-23 expression dramatically reduced the numbers of eosinophils and neutrophils in BALF and mitigated inflammation in the lungs of asthmatic mice. Furthermore, knockdown of IL-23 expression significantly decreased the levels of serum IgE, IL-23, IL-17, and IL-4, but not IFNgamma, and its anti-inflammatory effects were similar to or better than that of treatment with budesonide in asthmatic mice. Our data support the notion that IL-23 and associated Th17 responses contribute to the pathogenic process of bronchial asthma. Knockdown of IL-23 by RNAi effectively inhibits asthmatic inflammation, which is associated with mitigating the production of IL-17 and IL-4 in asthmatic mice.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plasmides
/
Asthme
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Test ELISA
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Liquide de lavage bronchoalvéolaire
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Ovalbumine
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RT-PCR
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Petit ARN interférent
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Interférence par ARN
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Granulocytes éosinophiles
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Interleukine-23
Limites du sujet:
Animals
langue:
En
Texte intégral:
Experimental & Molecular Medicine
Année:
2011
Type:
Article