Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea
The Korean Journal of Parasitology
;
: 85-89, 2004.
Article
Dans Anglais
| WPRIM
| ID: wpr-188031
ABSTRACT
Cryptosporidium parvum oocysts were isolated from a child suffering from acute gastroenteritis and successfully passaged in a calf and mice (designated hereafter SNU-H1) in the Republic of Korea; its molecular genotype has been analyzed. The GAG microsatellite region was amplified by a polymerase chain reaction (PCR), with a 238 base pair product, which is commonly displayed in C. parvum. The isolate was shown to be a mixture of the genotypes 1 (anthroponotic) and 2 (zoonotic). To study its infectivity in animals, 2 calves and 3 strains of mice were infected with the SNU-H1; in these animals, the propagation of both genotypes was successful. In immunosuppressed (ImSP) BALB/c and C57BL/6 mice the number of oocysts decreased after day 10 post-infection (PI) ; but in ImSP ICR mice, they remained constant until day 27 PI. The results show that both the C. parvum genotypes 1 and 2 can be propagated in calves and ImSP mice.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Zoonoses
/
Réaction de polymérisation en chaîne
/
Cryptosporidium parvum
/
Cryptosporidiose
/
Oocystes
/
Diarrhée
/
Fèces
/
Génotype
/
Corée
/
Souris de lignée BALB C
Limites du sujet:
Animaux
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2004
Type:
Article
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