An Aminopropyl Carbazole Derivative Induces Neurogenesis by Increasing Final Cell Division in Neural Stem Cells
Biomolecules & Therapeutics
;
: 313-319, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-189072
ABSTRACT
P7C3 and its derivatives, 1-(3,6-dibromo-9H-carbazol-9-yl)-3-(p-tolylamino)propan-2-ol (1) and N-(3-(3,6-dibromo-9H-carbazol-9-yl)-2-hydroxypropyl)-N-(3-methoxyphenyl)-4-methylbenzenesulfonamide (2), were previously reported to increase neurogenesis in rat neural stem cells (NSCs). Although P7C3 is known to increase neurogenesis by protecting newborn neurons, it is not known whether its derivatives also have protective effects to increase neurogenesis. In the current study, we examined how 1 induces neurogenesis. The treatment of 1 in NSCs increased numbers of cells in the absence of epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2), while not affecting those in the presence of growth factors. Compound 1 did not induce astrocytogenesis during NSC differentiation. 5-Bromo-2\'-deoxyuridine (BrdU) pulsing experiments showed that 1 significantly enhanced BrdU-positive neurons. Taken together, our data suggest that 1 promotes neurogenesis by the induction of final cell division during NSC differentiation.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Division cellulaire
/
Facteur de croissance fibroblastique de type 2
/
Maladies neurodégénératives
/
Protéines et peptides de signalisation intercellulaire
/
Facteur de croissance épidermique
/
Neurogenèse
/
Cellules souches neurales
/
Neurones
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Biomolecules & Therapeutics
Année:
2015
Type:
Article
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