Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence
The Korean Journal of Parasitology
;
: 299-304, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-190463
ABSTRACT
This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), beta-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Triose phosphate isomerase
/
Données de séquences moléculaires
/
ARN ribosomique 18S
/
Analyse de regroupements
/
Chine
/
Réaction de polymérisation en chaîne
/
ADN des protozoaires
/
Giardiase
/
Giardia lamblia
Limites du sujet:
Animaux
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2014
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS