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Acetoaminophen-induced accumulation of 8-oxodeoxyguanosine through reduction of Ogg1 DNA repair enzyme in C6 glioma cells
Experimental & Molecular Medicine ; : 71-77, 2004.
Article Dans Anglais | WPRIM | ID: wpr-190971
ABSTRACT
Large doses of acetaminophen (APAP) could cause oxidative stress and tissue damage through production of reactive oxygen/nitrogen (ROS/RNS) species and quinone metabolites of APAP. Although ROS/RNS are known to modify DNA, the effect of APAP on DNA modifications has not been studied systematically. In this study, we investigate whether large doses of APAP can modify the nuclear DNA in C6 glioma cells used as a model system, because these cells contain cytochrome P450-related enzymes responsible for APAP metabolism and subsequent toxicity (Geng and Strobel, 1995). Our results revealed that APAP produced ROS and significantly elevated the 8-oxo- deoxyguanosine (8-oxodG) levels in the nucleus of C6 glioma cells in a time and concentration dependent manner. APAP significantly reduced the 8- oxodG incision activity in the nucleus by decreasing the activity and content of a DNA repair enzyme, Ogg1. These results indicate that APAP in large doses can increase the 8-oxodG level partly through significant reduction of Ogg1 DNA repair enzyme.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Altération de l'ADN / ADN / Espèces réactives de l'oxygène / Analgésiques non narcotiques / Espèces réactives de l'azote / Lignée cellulaire tumorale / DNA Glycosylases / Désoxyguanosine / Réparation de l'ADN / Gliome Limites du sujet: Animaux / Humains langue: Anglais Texte intégral: Experimental & Molecular Medicine Année: 2004 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Altération de l'ADN / ADN / Espèces réactives de l'oxygène / Analgésiques non narcotiques / Espèces réactives de l'azote / Lignée cellulaire tumorale / DNA Glycosylases / Désoxyguanosine / Réparation de l'ADN / Gliome Limites du sujet: Animaux / Humains langue: Anglais Texte intégral: Experimental & Molecular Medicine Année: 2004 Type: Article