Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions
Journal of Veterinary Science
;
: 405-412, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-197113
ABSTRACT
The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor kappaB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0, 10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast differentiation and activation were monitored via tartrate-resistant acid phosphatase (TRAP) staining, filamentous-actin rings analysis, and a bone resorption assay. Furthermore, the expression osteoclast-related genes, such as TRAP and receptor activator for nuclear factor kappaB (RANK), that was influenced by OPG in RAW264.7 cells was examined using real-time polymerase chain reaction. In summary, findings from the present study suggested that M-CSF with RANKL can promote osteoclast differentiation and activation, and enhance the expression of TRAP and RANK mRNA in osteoclasts. In contrast, OPG inhibited these activities under serum-free conditions.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoclastes
/
Acid phosphatase
/
Cellules de la moelle osseuse
/
Cellules cultivées
/
Facteur de stimulation des colonies de macrophages
/
Protéines aviaires
/
Canards
/
Embryon non mammalien
/
Ligand de RANK
/
Récepteur activateur du facteur nucléaire Kappa B
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Journal of Veterinary Science
Année:
2013
Type:
Article
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