Usefulness of Enzyme-linked Immunosorbent Assay Using Recombinant BP180 and BP230 for Serodiagnosis and Monitoring Disease Activity of Bullous Pemphigoid
Annals of Dermatology
;
: 45-55, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-197937
ABSTRACT
BACKGROUND:
Bullous pemphigoid (BP) is an autoimmune subepidermal bullous disease associated with autoantibodies against BP180 and BP230. Enzyme-linked immunosorbent assay (ELISA) is a sensitive tool for the detection of immunoglobulin G (IgG) anti-BP180 and anti-BP230 autoantibodies.OBJECTIVE:
The aim of this study was to evaluate the usefulness of ELISA for diagnosing and monitoring the disease activity of BP.METHODS:
We evaluated serum IgG levels of anti-BP180 and anti-BP230 autoantibodies in 47 BP patients, 16 epidermolysis bullosa aquisita patients, and 15 healthy volunteers using ELISA. Through retrospective review of the medical records, the clinical characteristics of BP including disease activity, duration, pruritus severity and peripheral blood eosinophil counts were assessed.RESULTS:
The sensitivity of BP180 ELISA was 97.9%, BP230 ELISA 72.3%, and a combination of the two was 100%. The specificity of BP180 ELISA was 90.3%, BP230 ELISA 100%, and a combination of the two was 90.3%. BP180 ELISA scores showed strong associations with disease activity, pruritus severity, peripheral blood eosinophil counts, and disease duration, whereas BP230 ELISA scores did not.CONCLUSION:
BP180 and BP230 ELISAs are highly sensitive methods for the diagnosis of BP, and BP180 ELISA, in particular, is a sensitive tool for monitoring the disease activity of BP.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Prurit
/
Autoanticorps
/
Immunoglobuline G
/
Test ELISA
/
Tests sérologiques
/
Dossiers médicaux
/
Études rétrospectives
/
Épidermolyse bulleuse
/
Pemphigoïde bulleuse
/
Granulocytes éosinophiles
Type d'étude:
Etude diagnostique
/
Étude observationnelle
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Annals of Dermatology
Année:
2012
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS