Relationship between Microglial Activation and Dopaminergic Neuronal Loss in 6-OHDA-induced Parkinsonian Animal Model / 체질인류학회지
Korean Journal of Physical Anthropology
; : 13-23, 2013.
Article
Dans Ko
| WPRIM
| ID: wpr-199852
Responsable en Bibliothèque :
WPRO
ABSTRACT
This study assessed the dynamics of morphological and immunophenotypic properties of activated microglia in a 6-hydroxydopamine (6-OHDA) induced Parkinsonian animal model. Neurodegeneration in the substantia nigra pars compacta (SNc) was induced by unilateral injection of 6-OHDA into the medial forebrain bundle. Parkinsonian animal model were sacrificed at 1, 2, 4 and 8 weeks after 6-OHDA injection. Changes in the functional activity of activated microglia were identified using different monoclonal antibodies: OX6 for major histocompatibility complex (MHC) class II, ED1 for phagocytic activity. Phagocytic microglia, characterized by ED1- or OX6-immunoreactivity, appeared in the SNc at 1 week after 6-OHDA injection, activated microglia selectively adhered to degenerating axons, dendrites and dopaminergic neuron somas in the SNc. This was followed by significant loss of these fibers and nigral dopaminergic neurons. Activation of microglia into phagocytic stage was most pronounced at 2 week after 6-OHDA injection and gradually subsided, but phagocytic microglia persisted until 8 weeks after 6-OHDA injection. Taken together, our results indicate that activated microglia is lead to persistently neuron cell death and promotes loss of dopaminergic neuron by degeneration of the dopaminergic neurons.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Axones
/
Substantia nigra
/
Carisoprodol
/
Oxidopamine
/
Mort cellulaire
/
Microglie
/
Modèles animaux
/
Dendrites
/
Neurones dopaminergiques
/
Complexe majeur d'histocompatibilité
Limites du sujet:
Animals
langue:
Ko
Texte intégral:
Korean Journal of Physical Anthropology
Année:
2013
Type:
Article