Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model / 부인종양
Journal of Gynecologic Oncology
;
: 328-333, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-202217
ABSTRACT
OBJECTIVE:
To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment.METHODS:
Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 microg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30degrees C for hormone assays.RESULTS:
All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group.CONCLUSION:
G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Marqueurs biologiques
/
Facteur de stimulation des colonies de granulocytes
/
Cisplatine
/
Insuffisance ovarienne primitive
/
Rat Sprague-Dawley
/
Modèles animaux de maladie humaine
/
Évaluation préclinique de médicament
/
Hormone antimullérienne
/
Préservation de la fertilité
/
Follicule ovarique
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Journal of Gynecologic Oncology
Année:
2014
Type:
Article
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