Effects of Insulin-Like Growth Factor-1 on Proliferation of Human Corpus Cavernosal Smooth Muscle Cells / 대한남성과학회지
Korean Journal of Andrology
; : 18-22, 2003.
Article
de Ko
| WPRIM
| ID: wpr-203313
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: Insulin-like growth factor-1 (IGF-1) promotes the proliferation and migration of penile cavernous smooth muscle cells in the rat. The goals of this study were to evaluate the expression of IGF-1 and IGF-1 receptor (IGF-1R) in human corpus cavernosal smooth muscle cells (HCCSMCs) and to investigate the effect of IGF-1 on the proliferation of these cells in vitro. MATERIALS AND METHODS: The HCCSMCs were cultured from five impotent patients. The smooth muscle cells were identified by immunofluorescent stain. Total RNA was extracted, and IGF-1 gene expression was determined by RT-PCR. The IGF-1R immunoreactivity was examined by fluorescent Immunocytochemistry. Cell growth was assessed with a novel proliferation assay based on bioreduction of the fluorescent dye, Alamar blue. RESULTS: Endogenous IGF-1 mRNA expression was detected by RT-PCR analysis. The HCCSMCs were positive for IGF-IR. The proliferation of HCCSMCs increased with the dose in response IGF-1 in the culture medium in concentrations up to 100 ng/ml. CONCLUSIONS: Both IGF-1 and IGF-1R are expressed endogenously in human corpus cavernosal smooth muscle cells. IGF-1 promotes the proliferation of these cells in vitro.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
ARN
/
ARN messager
/
Facteur de croissance IGF-I
/
Immunohistochimie
/
Expression des gènes
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Récepteur IGF de type 1
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Myocytes du muscle lisse
/
Muscles lisses
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
/
Humans
langue:
Ko
Texte intégral:
Korean Journal of Andrology
Année:
2003
Type:
Article