Expression of TIM-3, Human beta-defensin-2, and FOXP3 and Correlation with Disease Activity in Pediatric Crohn's Disease with Infliximab Therapy
Gut and Liver
;
: 370-380, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-203889
ABSTRACT
BACKGROUND/AIMS:
This study investigated the expression of T cell immunoglobulin- and mucin-domain-containing molecule 3 (TIM-3), human beta-defensin (HBD)-2, forkhead box protein 3 (FOXP3), and the frequency of CD4+ CD25+ FOXP3+ regulatory T cells (Tregs) in children with Crohn's disease (CD) during infliximab therapy.METHODS:
We enrolled 20 CD patients who received infliximab treatment for 1 year. Peripheral blood and colonic mucosal specimens were collected from all CD patients and from healthy control individuals.RESULTS:
A significant difference in TIM-3 mRNA expression was evident in peripheral blood mononuclear cells and colonic mucosa between CD patients before infliximab therapy and the healthy controls (p<0.001 and p=0.005, respectively). A significant difference in HBD-2 mRNA expression was found in colonic mucosa between CD patients before infliximab therapy and the healthy controls (p=0.013). In the active phase of CD, at baseline, the median percentage of T cells that were CD25+ FOXP3+ was 1.5% (range, 0.32% to 3.49%), which increased after inflixmab treatment for 1 year to 2.2% (range, 0.54% to 5.02%) (p=0.008).CONCLUSIONS:
Our study suggests that both the adaptive and innate immune systems are closely linked to each other in CD pathogenesis. And the results of our study indicate that it could be a useful therapeutic tool, where restoration of TIM-3, HBD-2 and the function of Tregs may repair the dysfunctional immunoregulation in CD.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Agents gastro-intestinaux
/
Agranulocytes
/
Maladie de Crohn
/
Études cas-témoins
/
Lymphocytes T régulateurs
/
Côlon
/
Bêta-Défensines
/
Facteurs de transcription Forkhead
/
Infliximab
/
Muqueuse intestinale
Type d'étude:
Étude observationnelle
/
Facteurs de risque
Limites du sujet:
Adolescent
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Gut and Liver
Année:
2015
Type:
Article
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