The effects of beta-TCP/rhBMP-2 on bone formation in osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells
Journal of the Korean Association of Oral and Maxillofacial Surgeons
;
: 419-427, 2008.
Article
Dans Coréen
| WPRIM
| ID: wpr-205958
ABSTRACT
The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on beta-tricalcium phosphate (beta-TCP) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM beta-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor kappaB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-I (COL-I). There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on beta-TCP(+/-). According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/beta-TCP(-) than DOCs/beta-TCP(+). According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/beta-TCP(+) compared to that of DOCs/beta-TCP(-) on rhBMP 10 ng/ml. Our study presented the beta-TCP will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Ostéogenèse
/
Acide ascorbique
/
Facteurs de transcription
/
Phosphates de calcium
/
ARN messager
/
Dexaméthasone
/
Ostéocalcine
/
Calcium
/
Anthraquinones
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Journal of the Korean Association of Oral and Maxillofacial Surgeons
Année:
2008
Type:
Article
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