Analysis of Matrix Metalloproteinase Expression in the Recurrence of Superficial Low Grade Urothelial Bladder Cancer / 대한비뇨기과학회지
Korean Journal of Urology
; : 327-335, 2005.
Article
de Ko
| WPRIM
| ID: wpr-209459
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: Superficial bladder transitional cell carcinomas (TCCa) have high recurrence rates following endoscopic resection. Most of the parameters used for the prediction of a recurrence are associated with high grade TCCa. However, there are few valid parameters for prediction of the recurrence of low grade TCCa. We aimed to elucidate the prime factor of a recurrence of superficial low grade bladder TCCa. MATERIALS AND METHODS: Thirty cases of superficial low grade bladder TCCa were analyzed, 15 of which were recurred cases. Fresh frozen tumor and normal tissues were used for the analysis. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) and Western blots analyses were subsequently performed to evaluate the validity of the molecules identified by a 0.12K cDNA array, including matrix metalloproteinase (MMP), oncogenes and cell-cycle-related genes. RESULTS: On cDNA microarray analysis, MMP-1, 2, 9, 12 and 15, and transforming growth factor beta1 (TGFbeta1), vascular endothelial growth factor (VEGF) and fos were found to be significantly upregulated in the recurred cases. From the real-time RT-PCR for the validation of those molecules, MMP-1 and 12, and TGFbeta1, VEGF and fos were significantly overexpressed in the recurred cases. Western blot showed significant overexpression of MMP-1 and 12, and TGFbeta1, VEGF and fos in the recurred cases. CONCLUSIONS: The overexpressions of MMP-1 and 12, and TGFbeta1, VEGF and fos may be associated with the recurrence of superficial low grade bladder TCCa.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Oncogènes
/
Récidive
/
Vessie urinaire
/
Tumeurs de la vessie urinaire
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Carcinome transitionnel
/
Technique de Western
/
RT-PCR
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Séquençage par oligonucléotides en batterie
/
Matrix metalloproteinases
/
Facteur de croissance endothéliale vasculaire de type A
Type d'étude:
Prognostic_studies
langue:
Ko
Texte intégral:
Korean Journal of Urology
Année:
2005
Type:
Article