Proliferation of Corneal Endothelial Cells by Delivery of SV40 Large T Antigen
Journal of the Korean Ophthalmological Society
;
: 1331-1335, 2001.
Article
Dans Coréen
| WPRIM
| ID: wpr-209889
ABSTRACT
PURPOSE:
To determine whether the delivery of the SV40 large T-antigen is a feasible method for transiently inducing proliferation of corneal endothelial cells, we delivered liposome-protein complex into bovine corneal endothelial cells(BCEC).METHOD:
SV40 large T-antigen protein was introduced into BCEC and positive cells were identified by immunohistochemistry. Quiescent BCECs were double-labeled using BrdU as a measure of de novo DNA synthesis and the Ki-67 was detected by standard immunohistochemical methods.RESULT:
The treatment of quiescent BCECs with large T antigen caused an increase in BrdU incorporation and Ki-67 expression. It was tested by time-course study.CONCLUSION:
This finding suggests that liposome-mediated delivery of transforming proteins could be a method to transiently induce corneal endothelial cell proliferation.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Broxuridine
/
ADN
/
Immunohistochimie
/
Cellules endothéliales
/
Prolifération cellulaire
/
Antigènes des virus oncogènes
Type d'étude:
Étude pronostique
langue:
Coréen
Texte intégral:
Journal of the Korean Ophthalmological Society
Année:
2001
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS