Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening
The Korean Journal of Parasitology
;
: 21-26, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-210971
ABSTRACT
Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines de fusion recombinantes
/
Anticorps antihelminthe
/
Données de séquences moléculaires
/
Marqueurs biologiques tumoraux
/
Expression des gènes
/
Clonage moléculaire
/
Analyse de séquence d'ADN
/
Structures anatomiques de l'animal
/
Dirofilaria immitis
/
Modèles animaux de maladie humaine
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2014
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS