Isolation of Phenolate Type Siderophore from Pseudomonas sp. PY002
Journal of the Korean Society for Microbiology
;
: 39-46, 1997.
Article
Dans Coréen
| WPRIM
| ID: wpr-211420
ABSTRACT
Phenolate type siderophore was produced in Pseudomonas sp. PY002 (P. sp. PY002) which cultured in M9 minimal medium supplemented with 0 to 500 uM of dipyridyl. Pyochelin, a kind of siderophore, was detected as a single broad absorption band (280 nm) at pH 12.0, which is a characteristic of phenolate type siderophore. The 280 nm absorption spectrum of pyochelin was changed to 310 nm at pH 1.5. The pyochelin produced was a structurally unique phenolate siderophore, designated 2-[2-(o-hydroxyphenyl)-2-thiazolin-4-yl]-3-methyl-4-thiazolidine car- boxylic acid on the analysis of infrared radiation and 'H and ""C nuclear magnetic resonance spectroscopy. Additionally, purified pyochelin increased the cell growth rate, like as growth fac- tor. All these results suggest that phenolate type siderophore play an important role in cell growth of P. sp. PY002.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pseudomonas
/
Analyse spectrale
/
Spectroscopie par résonance magnétique
/
Phénol
/
Absorption
/
Concentration en ions d'hydrogène
langue:
Coréen
Texte intégral:
Journal of the Korean Society for Microbiology
Année:
1997
Type:
Article
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