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Flow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive Calcium Channel
Article de En | WPRIM | ID: wpr-212103
Bibliothèque responsable: WPRO
ABSTRACT
Radial glial cells (RGCs) which function as neural stem cells are known to be non-excitable and their proliferation depends on the intracellular calcium (Ca²⁺) level. It has been well established that Inositol 1,4,5-trisphosphate (IP3)-mediated Ca²⁺ release and Ca²⁺ entry through various Ca²⁺ channels are involved in the proliferation of RGCs. Furthermore, RGCs line the ventricular wall and are exposed to a shear stress due to a physical contact with the cerebrospinal fluid (CSF). However, little is known about how the Ca²⁺ entry through mechanosensitive ion channels affects the proliferation of RGCs. Hence, we hypothesized that shear stress due to a flow of CSF boosts the proliferative potential of RGCs possibly via an activation of mechanosensitive Ca²⁺ channel during the embryonic brain development. Here, we developed a new microfluidic two-dimensional culture system to establish a link between the flow shear stress and the proliferative activity of cultured RGCs. Using this microfluidic device, we successfully visualized the artificial CSF and RGCs in direct contact and found a significant enhancement of proliferative capacity of RGCs in response to increased shear stress. To determine if there are any mechanosensitive ion channels involved, a mechanical stimulation by poking was given to individual RGCs. We found that a poking on radial glial cell induced an increase in intracellular Ca²⁺ level, which disappeared under the extracellular Ca²⁺-free condition. Our results suggest that the shear stress by CSF flow possibly activates mechanosensitive Ca²⁺ channels, which gives rise to a Ca²⁺ entry which enhances the proliferative capacity of RGCs.
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Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Encéphale / Canaux calciques / Inositol 1,4,5-trisphosphate / Liquide cérébrospinal / Calcium / Microfluidique / Laboratoires sur puces / Cellules souches neurales / Cellules épendymogliales / Canaux ioniques langue: En Texte intégral: Experimental Neurobiology Année: 2017 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Encéphale / Canaux calciques / Inositol 1,4,5-trisphosphate / Liquide cérébrospinal / Calcium / Microfluidique / Laboratoires sur puces / Cellules souches neurales / Cellules épendymogliales / Canaux ioniques langue: En Texte intégral: Experimental Neurobiology Année: 2017 Type: Article