Detection of Serum Anti-p53 Antibodies and Their Significance in Patients with Colorectal Cancer / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 168-173, 2005.
Article
Dans Coréen
| WPRIM
| ID: wpr-214447
ABSTRACT
BACKGROUND:
Mutations of the tumor suppressor gene p53 cause subsequent cellular accumulation of p53 gene product and a specific immunologic response. Detection of circulating antibodies against p53 protein has been evaluated for a tumor marker or prognostic factor for several cancers including those of the breast, stomach, ovary and lung.METHODS:
Serum samples from 74 colorectal cancer patients were obtained preoperatively and anti-p53 antibody was measured by enzyme immunoassay (Anti-P53 ELISA II PharmaCell, France). Carcinoembryonic antigen (CEA) and Carbohydrate antigen (CA) 19-9 were measured in parallel. Tissue p53 protein expression was examined by immunohistochemical staining.RESULTS:
Anti-p53 antibodies were positive in the serum from 34% (25/74) of patients, but normal controls were all negative. Anti-p53 antibodies were significantly associated with p53 protein overexpression. CEA and CA19-9 were detected in 38% and 11%, respectively. There was no correlation among anti-p53 antibody, CEA and CA19-9. No differences were found between the anti-p53 antibody positive and negative groups in the following parameters tumor site, histologic grades, Dukes stages, margin invasion, vessel invasion, lymph node and distant metastasis, and survival duration.CONCLUSIONS:
The measurement of serum anti-p53 antibody is not suitable for preoperative markers of prognostic significance, but can be used as a simple serological marker for detection of p53 alteration. There should be more studies of the serum p53 antibody as a possible marker for postoperative monitoring in colorectal cancer.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ovaire
/
Estomac
/
Région mammaire
/
Test ELISA
/
Tumeurs colorectales
/
Antigène carcinoembryonnaire
/
Gènes p53
/
Gènes suppresseurs de tumeur
/
Techniques immunoenzymatiques
/
Poumon
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Femelle
/
Humains
langue:
Coréen
Texte intégral:
The Korean Journal of Laboratory Medicine
Année:
2005
Type:
Article
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