Effect of Platelet-rich Plasma(PRP) on Regeneration of Rat Sciatic Nerve in a Silicone Chamber

ABSTRACT

PURPOSE: The purpose of this study is to determine the effect of platelet-rich plasma(PRP) on rat sciatic nerve regeneration in a 10mm silicone chamber. METHODS: A total of 6 inbred Lewis rats were used in this study. Bilateral sciatic neurectomy was performed on each rat. On one side, silicone chambers containing PRP solutions were implanted; on the contralateral side, the chambers without PRP were implanted as a control. In 12 weeks post-implantation, chambers were retrieved and both gastrocnemius muscles were excised. Nerves biopsy samples were examined under a light microscope after Masson trichrome staining. RESULTS: Cross sections of the midpoints of PRP treated nerves were significantly larger and appeared more mature than those of controls. CONCLUSION: Based on morphological evidence, PRP has a positive effect on neural regeneration, and it may therefore be useful for treating peripheral nerve injuries.