Quantitative Analysis of Eleven Bioactive Constituents of a Traditional Herbal Medicine, Yeonggyechulgam-tang using, Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry
Natural Product Sciences
; : 84-91, 2017.
Article
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| WPRIM
| ID: wpr-216624
Responsable en Bibliothèque :
WPRO
ABSTRACT
This study proposes a sensitive and selective liquid chromatography-electrospray ionization tandem mass spectrometry method of efficiently assessing the quality of a traditional herbal medicine called Yeonggyechulgam-tang (YGCGT). The following compounds 1 – 11, namely, liquiritin apioside (1), liquiritin (2), liquiritigene (3), coumarin (4), cinnamic acid (5), cinnamaldehyde (6), glycyrrhizin (7), atractylenolide III (8), atractylenolide II (9), atractylenolide I (10), and pachymic acid (11) were separated on a UPLC BEH C₁₈ column (2.1 × 100 mm, 1.7 µm) at a column temperature of 45℃ eluted with a gradient condition of 0.1% (v/v) formic acid in distilled water and acetonitrile. The correlation coefficient of the calibration curve of the eleven constituents was ≥ 0.9936. The limits of detection and quantification of the compounds 1 – 11 were 0.06 – 4.73 ng/mL and 0.17–14.20 ng/mL, respectively. Using this analytical method, the compound 11 in lyophilized YGCGT decoction extract was not detected, while the compounds 1 – 10 were detected 0.13–166.43 mg/g.
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Calibrage
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Eau
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Acide glycyrrhizique
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Science des plantes médicinales
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Spectrométrie de masse en tandem
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Limite de détection
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Méthodes
langue:
En
Texte intégral:
Natural Product Sciences
Année:
2017
Type:
Article