Effect of recombinant human bone morphogenetic protein-2 on bisphosphonate-treated osteoblasts
Journal of the Korean Association of Oral and Maxillofacial Surgeons
;
: 291-296, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-222018
ABSTRACT
OBJECTIVES:
Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a side effect of bisphophonate therapy that has been reported in recent years. Osteoclastic inactivity by bisphosphonate is the known cause of BRONJ. Bone morphogenetic protein-2 (BMP-2) plays an important role in the development of bone. Recombinant human BMP-2 (rhBMP-2) is potentially useful as an activation factor for bone repair. We hypothesized that rhBMP-2 would enhance the osteoclast-osteoblast interaction related to bone remodeling. MATERIALS ANDMETHODS:
Human fetal osteoblast cells (hFOB 1.19) were treated with 100 microM alendronate, and 100 ng/mL rhBMP-2 was added. Cells were incubated for a further 48 hours, and cell viability was measured using an MTT assay. Expression of the three cytokines from osteoblasts, receptor activator of nuclear factor-kappaB ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF), were analyzed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS:
Cell viability was decreased to 82.75%+/-1.00% by alendronate and then increased to 110.43%+/-1.35% after treatment with rhBMP-2 (P<0.05, respectively). OPG, RANKL, and M-CSF expression were all decreased by alendronate treatment. RANKL and M-CSF expression were increased, but OPG was not significantly affected by rhBMP-2.CONCLUSION:
rhBMP2 does not affect OPG gene expression in hFOB, but it may increase RANKL and M-CSF gene expression.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Ostéoclastes
/
Test ELISA
/
Expression des gènes
/
Survie cellulaire
/
Cytokines
/
Facteur de stimulation des colonies de macrophages
/
Remodelage osseux
/
Alendronate
/
Ligand de RANK
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Journal of the Korean Association of Oral and Maxillofacial Surgeons
Année:
2014
Type:
Article
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