miR-98 suppresses melanoma metastasis through a negative feedback loop with its target gene IL-6
Experimental & Molecular Medicine
;
: e116-2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-222036
ABSTRACT
Dysregulated microRNA (miRNA) expression has a critical role in tumor development and metastasis. However, the mechanism by which miRNAs control melanoma metastasis is unknown. Here, we report reduced miR-98 expression in melanoma tissues with increasing tumor stage as well as metastasis; its expression is also negatively associated with melanoma patient survival. Furthermore, we demonstrate that miR-98 inhibits melanoma cell migration in vitro as well as metastatic tumor size in vivo. We also found that IL-6 is a target gene of miR-98, and IL-6 represses miR-98 levels via the Stat3-NF-kappaB-lin28B pathway. In an in vivo melanoma model, we demonstrate that miR-98 reduces melanoma metastasis and increases survival in part by reducing IL-6 levels; it also decreases Stat3 and p65 phosphorylation as well as lin28B mRNA levels. These results suggest that miR-98 inhibits melanoma metastasis in part through a novel miR-98-IL-6-negative feedback loop.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Transduction du signal
/
Régulation négative
/
Régulation de l'expression des gènes tumoraux
/
Analyse de survie
/
Interleukine-6
/
MicroARN
/
Lignée cellulaire tumorale
/
Mélanome
/
Souris de lignée C57BL
/
Métastase tumorale
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Experimental & Molecular Medicine
Année:
2014
Type:
Article
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