The effects of chromium exposure on sister chromatid exchange and concentration of 8-hydroxydeoxyguanosine / 예방의학회지
Korean Journal of Preventive Medicine
; : 511-525, 1995.
Article
de Ko
| WPRIM
| ID: wpr-224027
Bibliothèque responsable:
WPRO
ABSTRACT
To elucidate some DNA adducts as a biological marker for workers of chromate pigment, the effects of chromium exposure on the formation of 8-hydroxydeoxyguanosine(8-OH-dG) and sister chromatid exchanges(SCEs) frequency in 38 workers of a pigment plant in Bucheon which utilized lead chromates, were examined. The chromium contents of venous blood and urine were measured as working environmental exposure level. The concentrations of 8-OH-dG in DNA isolated from lymphocytes were determined with high performance liquid chromatography and electrochemical detector and denoted as a molar ratio of 8-OH-dG to deoxyguanosine(dG). The SCEs frequency were analyzed in DNA isolated from lymphocytes. A significant correlation was found between creatinine adjusted urine chromium concentration and the molar ratio of 8-OH-dG to dG(r=0.47, p<0.01). After adjusting the current smoking habit, the correlation coefficient was increased(r=0.62, p<0.05). However, there was no significant correlation between the SCE frequency and chromium exposure. This significant results between molar ratio of 8-OH-dG to dG and chromium exposure are in good agreement with in vitro studies that support the importance of DNA adduct formation for the carcinogenic effect of chromium.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plantes
/
Échange de chromatides soeurs
/
Fumée
/
ADN
/
Lymphocytes
/
Marqueurs biologiques
/
Fumer
/
Chromates
/
Chromatides
/
Chromatographie en phase liquide
Limites du sujet:
Humans
langue:
Ko
Texte intégral:
Korean Journal of Preventive Medicine
Année:
1995
Type:
Article