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Kappa-opioid receptor activation during reperfusion limits myocardial infarction via ERK1/2 activation in isolated rat hearts / 대한마취과학회지
Article de En | WPRIM | ID: wpr-224612
Bibliothèque responsable: WPRO
ABSTRACT
BACKGROUND: We investigated whether p42/p44 extracellular signal-regulated kinases (ERK1/2) and/or phosphatidylinositol-3-OH kinase (PI3K)-Akt play a crucial role in cardioprotection by kappa-opioid receptor (KOP) activation. METHODS: Langendorff perfused rat hearts were subjected to 30 min of regional ischemia and 2 h of reperfusion. Antagonists of ERK1/2 and PI3K were perfused in hearts treated with the KOP agonist U50488H (U50). Infarct size was measured after 2 h of reperfusion. The phosphorylation states of ERK1/2 and Akt by Western immunoblots were determined. Drugs were perfused for a period of 5 min before and 30 min after reperfusion. RESULTS: Inhibition of ERK1/2 (26.8 +/- 2.9%, P 0.05 vs. U50) completely abrogated the anti-infarct effect of U50488H. Western blot analysis revealed a significant increase in ERK1/2 but not Akt phsophorylation in U50488H-treated hearts as compared to control hearts when measured immediately after reperfusion. CONCLUSIONS: KOP activation effectively reduces myocardial infarction. The anti-infarct effect of U50488H is mediated by the ERK1/2, but not the PI3K-Akt pathway.
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Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Phosphorylation / Reperfusion / Technique de Western / Récepteurs aux opioïdes / 2-(3,4-Dichlorophényl)-N-méthyl-N-((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)acétamide / Phosphatidylinositol 3-kinases / Extracellular Signal-Regulated MAP Kinases / Occlusion coronarienne / Coeur / Ischémie Limites du sujet: Animals langue: En Texte intégral: Korean Journal of Anesthesiology Année: 2011 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Phosphorylation / Reperfusion / Technique de Western / Récepteurs aux opioïdes / 2-(3,4-Dichlorophényl)-N-méthyl-N-((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)acétamide / Phosphatidylinositol 3-kinases / Extracellular Signal-Regulated MAP Kinases / Occlusion coronarienne / Coeur / Ischémie Limites du sujet: Animals langue: En Texte intégral: Korean Journal of Anesthesiology Année: 2011 Type: Article