Genetic Variation of SCN5A in Korean Patients with Sick Sinus Syndrome
Korean Circulation Journal
;
: 63-71, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-22789
ABSTRACT
BACKGROUND AND OBJECTIVES:
Due to recent studies that have shown an association between the genetic variation of SCN5A and sick sinus syndrome (SSS), we sought to determine if a similar correlation existed in Korean patients with SSS. SUBJECTS ANDMETHODS:
We enrolled 30 patients with SSS who showed a sinus pause (longer than 3.0 s) in Holter monitoring, in addition to 80 controls. All exons including the putative splicing sites of the SCN5A gene were amplified by polymerase chain reaction and sequenced either directly or following subcloning. Wild-type and single nucleotide polymorphisms were expressed in human embryonic kidney cells, and the peak sodium current (I(Na)) was analyzed using the whole-cell patch-clamp technique.RESULTS:
A total of 9 genetic variations were identified 7 variations (G87A-A29A, IVS9-3C>A, A1673G-H558R, G3823A-D1275N, T5457C-D1819D, T5963G-L1988R, and C5129T-S1710L) had been previously reported, and 2 variants (A3075T-E1025D and T4847A-F1616Y) were novel; the potential structural effects of F1616Y were analyzed in a three-dimensional model of the SCN5A domain. Patch-clamp studies at room temperature demonstrated that the peak I(Na) was significantly increased by 140% in HEK cells transfected with F1616Y compared with wild-type (-335.13 pA/pF+/-24.04, n=8 vs. -139.95 pA/pF+/-23.76, n=7, respectively). Furthermore, the voltage dependency of the activation and steady-state inactivation of F1616Y were leftward-shifted compared with wild-type (V(h) activation=-55.36 mv+/-0.22, n=8 vs. V(h) activation=-44.21 mV+/-0.17, n=7; respectively; V(h) inactivation=-104.47 mV+/-0.21, n=7 vs. V(h) inactivation=-84.89 mV+/-0.09, n=12, respectively).CONCLUSION:
F1616Y may be associated with SSS.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Maladie du sinus
/
Sodium
/
Variation génétique
/
Réaction de polymérisation en chaîne
/
Exons
/
Électrocardiographie ambulatoire
/
Techniques de patch-clamp
/
Polymorphisme de nucléotide simple
/
Rein
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Korean Circulation Journal
Année:
2016
Type:
Article
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