Establishment of cell free conversion system with biotin-labelled recombinant PrPsen expressed in E. coli / 生物医学与环境科学（英文）

ABSTRACT

<p><b>OBJECTIVE</b>To report a protocol using biotin-labelled PrP protein in cell free conversion assay instead of isotope.</p><p><b>METHODS</b>A hamster PrP protein (HaPrP) was expressed in E. coli and purified with HIS-tag affinity chromatograph. After being labelled with biotin, HaPrP was mixed with PrPSc preparation from scrapie strain 263K.</p><p><b>RESULTS</b>Protease-resistant bands were detected after four-day incubation.</p><p><b>CONCLUSION</b>The new conversion model provides a reliable, easily handling, and environment-friendly method for studies of prion and transmissible spongiform encephalopathies.</p>