Studies of chemical constituents and their antioxidant activities from Astragalus mongholicus Bunge / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 297-301, 2005.
Article
Dans Anglais
| WPRIM
| ID: wpr-229752
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the antioxidant activities of different chemical constituents from Astragalus mongholicus Bunge and their protection against xanthine (XA)/xanthine oxidase (XO)-induced toxicity in PC12 cells.</p><p><b>METHODS</b>The compounds of Astragalus mongholicus Bunge were isolated by chromatography and the structures were elucidated on the basis of spectral data interpretation. Their antioxidant activities were detected by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities in a cell-free system. Meanwhile, the effects against XA/XO-induced toxicity were assessed using MTT assay in PC12 cells.</p><p><b>RESULTS</b>Ten principal constituents were isolated and identified as formononetin (I), ononin (II), calycosin (III), calycosin-7-O-beta-D-glucoside (IV), 9,10-dimethoxypterocarpan-3-O-beta-D-glucoside (V), adenosine (VI), pinitol (VII), daucosterol (VIII), beta-sitoster (IX) and saccharose (X) from Astragalus mongholicus Bunge. The compounds I, III, and IV scavenged DPPH free radicals in vitro. Formononetin and calycosin were found to inhibit XA/XO-induced cell injury significantly, with an estimated EC50 of 50 ng/mL.</p><p><b>CONCLUSION</b>Compound II, VI, and VII are first reported in this plant. Calycosin exhibits the most potent antioxidant activity both in the cell-free system and in the cell system.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Xanthine oxidase
/
Médicaments issus de plantes chinoises
/
Chimie
/
Piégeurs de radicaux libres
/
Cellules PC12
/
Xanthine
/
Astragalus
/
Toxicité
/
Radicaux libres
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2005
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS