Detection of Y chromosome microdeletions in AZF region by liquid chip technology / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 86-88, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-229813
ABSTRACT
<p><b>OBJECTIVE</b>To establish a liquid chip technology to detect Y chromosome microdeletions in Chinese infertile males with azoospermia or oligozoospermia.</p><p><b>METHODS</b>Multiplex PCR and liquid chip technology were used to detect the Y chromosome microdeletions in AZF region in 178 infertile patients with azoospermia and 134 infertile patients with oligozoospermia as well as 40 fertile control men.</p><p><b>RESULTS</b>Forty out of 312 patients (12.8%) were found to have deletions in AZF region. The microdeletion frequency was 14%(25/178) in the azoospermic group, 9.6%(11/114) in the oligospermic and 20%(4/20) in the severe oligospermic group.</p><p><b>CONCLUSION</b>The authors developed a high-throughput, fast and simple assay to screen the AZF region microdeletions of Y chromosome.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oligospermie
/
Séquence nucléotidique
/
Sites étiquetés par des séquences
/
Délétion de segment de chromosome
/
Séquençage par oligonucléotides en batterie
/
Chromosomes Y humains
/
Asiatiques
/
Électrophorèse sur gel de polyacrylamide
/
Azoospermie
/
Génétique
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2008
Type:
Article
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