Differentiation of rat bone marrow derived Thy-1+ beta2M- cells into hepatocytes / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 69-73, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-229904
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differentiation of bone marrow derived Thy-1(+)beta(2)M(-) cells (BDTC) into mature and functional liver cells and its mechanism.</p><p><b>METHODS</b>BDTC were cocultured with allyl alcohol (AA)-injured hepatocytes and cultured alone in conditional medium containing HGF and bFGF, respectively. BDTC morphologic transformation was observed with phase-contrast and electron-microscopy. Hepatocyte-specific gene expression in cultured BDTC was identified by immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Indocyanine green (ICG) ingestion/excretion and urea, albumin production were carried out to evaluate hepatocyte-related function.</p><p><b>RESULTS</b>Some BDTC derived hepatocyte-like cells with high nuclear to cytoplasmic ratio containing mono- or multi-nuclei and abundant mitochondria, endoplasmic reticulum and glycogenic granules appeared after 7-day culture in both the two culture systems. These cells expressed hepatocyte-specific genes (AFP, OV-6, CK18, etc.), and possessed functions of ICG uptake, albumin production and ammonium metabolism.</p><p><b>CONCLUSION</b>Rat BDTCs could differentiate into mature and functional liver cells in special stimulation systems. Moreover, these differentiations were realized by "transdifferentiation", and might dispense with "cell fusion".</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Cellules de la moelle osseuse
/
Immunohistochimie
/
Alphafoetoprotéines
/
Expression des gènes
/
Différenciation cellulaire
/
Cellules cultivées
/
Facteur de croissance fibroblastique de type 2
/
Rat Wistar
/
Facteur de croissance des hépatocytes
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2005
Type:
Article
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