Influence of rhG-CSF on activity of sphingosine kinase in monocytes / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 156-159, 2007.
Article
Dans Zh
| WPRIM
| ID: wpr-230311
Responsable en Bibliothèque :
WPRO
ABSTRACT
The aim of this research was to understand the influence of rhG-CSF on the sphingosine kinase (SphK) activity of monocytes. The peripheral blood monocytes were collected from 6 peripheral blood progenitor cell donors on the fifth day of mobilization with rhG-CSF and from 5 blood donors' buffy coats. The mRNA expressions of monocyte G-CSF receptor and SphK were tested with RT-PCR. The changes of SphK activity of monocytes were assayed after being treated with rhG-CSF. The results showed that the two kinds monocytes collected from both blood donors and peripheral blood progenitor cell donors mobilized with rhG-CSF expressed mRNA of G-CSF receptor and SphK. The SphK activity of monocytes collected from blood donors was not changed significantly after being treated with rhG-CSF (P > 0.05). The SphK activity of monocytes collected from peripheral blood progenitor cell donors transiently increased by (39.6 - 87.2)% after being treated by means of rhG-CSF (P < 0.05) without obviously dose-dependent effect. It is concluded that the SphK activity of monocytes collected from peripheral blood progenitor cell donors can be activated by rhG-CSF.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Protéines recombinantes
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Monocytes
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Facteur de stimulation des colonies de granulocytes
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Récepteur de facteur de croissance granulocytaire
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Phosphotransferases (Alcohol Group Acceptor)
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Mobilisation de cellules souches hématopoïétiques
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Biologie cellulaire
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Génétique
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Métabolisme
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2007
Type:
Article