Investigation of canine mesenchymal stem cells differentiation to vascular endothelial cell in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1348-1351, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-230688
ABSTRACT
To induce endothelial cell, canine bone marrow-derived mesenchymal stem cells (MSCs) were separated from bone marrow by density gradient centrifugation. The isolated MSCs were induced to form endothelial-like cell in the presence of vascular endothelial growth factor (VEGF), endothelial growth factor (EGF) and so on. These results showed that the cells uniformly took on a cobblestone morphology under the light microscope, and cell nucleolus was in the middle of the cells. The cells displayed Weibel-Palade bodies under the transmission electron microscope. vWF, a specific marker of endothelial cell was positive in the cells. The above results demonstrate that MSCs may be differentiated into endothelial cells in vitro.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Cellules de la moelle osseuse
/
Différenciation cellulaire
/
Cellules cultivées
/
Facteurs de croissance endothéliale
/
Techniques de culture cellulaire
/
Biologie cellulaire
/
Cellules endothéliales
/
Facteur de croissance endothéliale vasculaire de type A
/
Cellules souches mésenchymateuses
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Biomedical Engineering
Année:
2007
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS