In vitro study for detecting the effects of Bazhen decoction on proliferation and activation of T lymphocytes / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 855-858, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-230770
ABSTRACT
This study was designed to detect the effects of Bazhen decoction on the proliferation and activation of T lymphocytes. T lymphocytes were isolated. The effects of Bazhen decoction on the prolifertion and activation of T lymphocytes, and on the secretion of IFN-gamma IL-2 from T lymphocytes, were detected by MTT, Flow cytometry and ELISA. Results showed that proliferation of T lymphocytes was promoted significantly by different concentration of Bazhen decoction; and after different time, the relationships of "the longer the time, the higher the concentration, and the more enhanced the proliferation" came to be apparent. After 72h, T lymphocytes were activated with different concentration of Bazhen decoction, the rate of CD69+ T cells increased signficantly, the secretion of IFN-gamma and IL-2 increased signficicantly, and the effect exhibited a dose-dependent manner. The results of this epxeriment indicated that Bazhen decoction could promote the proliferation and activation of T lymphocytes.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Médicaments issus de plantes chinoises
/
Activation des lymphocytes
/
Lymphocytes T
/
Cellules cultivées
/
Biologie cellulaire
/
Prolifération cellulaire
/
Allergie et immunologie
/
Cytométrie en flux
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Biomedical Engineering
Année:
2010
Type:
Article
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