Lipid-peroxidantion damage of embryo and placenta induced by artesunate in rats / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 41-45, 2003.
Article
de Zh
| WPRIM
| ID: wpr-231125
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the effect and mechanism of artesunate (Art) on embryo development.</p><p><b>METHODS</b>Rat embryo and placental glutathione peroxidase (GSH-Px)and malondialdehyde (MDA) were identified by using DTNB (dithionitrobenzene) direct method and TBA (thiobarbituric acid). We investigated the damage of decidual cells caused by Art using cell culture techniques.</p><p><b>RESULTS</b>Subcutaneous administration of Art in rats on d 6 approximate, equals d 10 of gestation induced developmental toxicity. Absorption increased when progressively increased doses were given (r=0.996,P<0.01). Twenty four hours post injection, GSH-Px in embryo decreased significantly while MDA content was significantly higher than that in the control group (P<0.05). GSH Px: study group was(43.7+/-10.7)micromol/min.mg(-1)Hb, control group was(54.5+/-10.1)micromol/min.mg(-1)Hb; MDA:study group was(230.2+/-19.8)nmol/g tissue, control group was(150.4+/-44.1)nmol/g tissue. Placental GSH-Px activity was significantly higher than that in the control group(P<0.01). After cultured human decidual cells were exposed to Art for 24 h, the LC50 was (25.2+/-3.5)mg/L.</p><p><b>CONCLUSION</b>Art may induce developmental toxicity in rat embryo and placenta by neutralizing the antioxidant defense mechanism.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Placenta
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Sesquiterpènes
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Peroxydation lipidique
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Rat Sprague-Dawley
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Artémisinines
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Embryon de mammifère
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Toxicité
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Glutathione peroxidase
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Métabolisme
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Antipaludiques
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2003
Type:
Article