Inhibition of telomerase with hTERT antisense enhances TNF-alpha-induced apoptosis in prostate cancer cells PC3 / 中华男科学杂志
National Journal of Andrology
;
(12): 723-726, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-232076
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibiting effect of telomerase with hTERT antisense on TNF-alpha-induced apoptosis in prostate cancer cells PC3.</p><p><b>METHODS</b>Antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomeric repeat amplification protocol (TRAP) and telomerase PCR-ELISA Kit, cell viability was determined by MTT assay, and cell apoptosis was observed by morphological method and determined by flow cytometry.</p><p><b>RESULTS</b>AS PS-ODN could significantly inhibit the telomerase activity and increase the susceptibility of TNF-alpha-induced apoptosis of PC3 cells.</p><p><b>CONCLUSION</b>Inhibition of telomerase with hTERT antisense can enhance TNF-alpha-induced apoptosis in prostate cancer cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Tumeurs de la prostate
/
Test ELISA
/
Survie cellulaire
/
Réaction de polymérisation en chaîne
/
Facteur de nécrose tumorale alpha
/
Apoptose
/
Telomerase
/
Oligodésoxyribonucléotides antisens
Limites du sujet:
Humains
/
Mâle
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2007
Type:
Article
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