Significance of detecting free DNA from maternal plasma for the diagnosis of fetal chromosomal aneuploidies / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 435-438, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-232281
ABSTRACT
<p><b>OBJECTIVE</b>To determine the feasibility and accuracy of detecting numerical chromosomal abnormalities by high-flux sequencing analysis of free fetal DNA from maternal plasma.</p><p><b>METHODS</b>High-flux sequencing was applied to analyze fetal chromosome sequence copy numbers in 153 pregnant women. Fetal karyotyping was also carried out on amniocentesis samples.</p><p><b>RESULTS</b>Six cases were detected with fetal chromosomal abnormalities by high-flux sequencing analysis, among which five were confirmed by karyotyping to be chromosomal aneuploidies (47,XYY; 45,X; 47,XY,+18; 47,XY,+21 and 47,XY,+13), 1 case was confirmed to be structural rearrangement, i.e., 46,XY,der(13;21)(q10;q10),+21. Furthermore, 3 chromosomal polymorphisms (one 46,XY,21p+ and two 46,XY,Yqh-) were identified. The two methods yielded similar results on fetal chromosome copy number detection.</p><p><b>CONCLUSION</b>High-flux sequencing analysis of free DNA derived from maternal plasma is efficient for detecting fetal chromosomal aneuploidies, and is non-invasive, highly sensitive and specific. It therefore has a broad application in antenatal diagnosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Diagnostic prénatal
/
ADN
/
Chimie
/
Maladies chromosomiques
/
Diagnostic
/
Foetus
/
Génétique
/
Amniocentèse
/
Aneuploïdie
/
Méthodes
Type d'étude:
Etude diagnostique
Limites du sujet:
Adulte
/
Femelle
/
Humains
/
Grossesse
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2012
Type:
Article
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