Effect of Jianpi Qinghua decoctions on interleukin-6 and monocyte chemotactic protein-1 in rats with focal segmental glomurular sclerosis nephropathy: role of the inflammatory signaling pathways / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of Jianpi Qinghua decoctions in ameliorating kidney fibrosis in rats with focal segmental glomurular sclerosis (FSGS) nephropathy in light of blocking the functions of the inflammatory cells.</p><p><b>METHODS</b>Rat models of FSGS nephropathy were established by left nephrectomy and intravenous injection of adriamycin and treated with Jianpi Qinghua decoction. The renal expressions of interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1), and intercellular adhesion molecular-1 (ICAM-1) were detected.</p><p><b>RESULTS</b>Treatment with Jianpi Qinghua decoction, Jianpi decoction, Qinghua decoction, and Niaoduqing particles all significantly lowered renal tubulointerstitial expressions of IL-6 in the model rats (P<0.01). Renal tubulointerstitial MCP-1 expressions were all lowered significantly in the rats by treatments with Jianpi Qinghua decoction, Jianpi decoction, QingHua decoctions, and Niaoduqing particles (P<0.01), and Jianpi Qinghua decoction produced a stronger effect than Niaoduqing particles (P<0.05). Jianpi Qinghua decoction, Jianpi decoction, Qinghua decoctions and and Niaoduqing particles all significantly decreased renal tubulointerstitial MCP-1 and ICAM-1 expressions (P<0.01), and Jianpi Qinghua and Qinghua decoctions both showed stronger effects than Niaoduqing particles (P<0.01). Jianpi Qinghua decoctions significantly suppressed the expressions of IL-6, MCP-1, and ICAM-1 and produced stronger effects than Niaoduqing particles on MCP-1 and ICAM-1 expressions.</p><p><b>CONCLUSION</b>Jianpi Qinghua decoctions can ameliorate inflammatory injury and lower the levels of inflammatory factors in rats with FSGS nephropathy, the mechanism of which is associated with inhibiting IL-6 signaling pathway.</p>