ITS sequence of 9 Bupleurum species and its application in identification of Chaihu (Radix Buplurei) / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1460-1463, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-232859
ABSTRACT
<p><b>OBJECTIVE</b>The study the patterns of rDNA ITS sequence variation of 9 medicinal species of genus Bupleurum in China to find the DNA molecular markers for identification of these crude drugs.</p><p><b>METHOD</b>The ITS regions of these species were amplified by PCR and sequenced, and their sequences were analyzed by DNAssist Version 2.2.</p><p><b>RESULTS</b>Homologous alignment indicated that the consanguinity of the ITS sequences between genus Bupleurum and outgroups was lower than 75%, and that within genus Bupleurum was higher than 88%. For the same species, the consanguinity was higher than 99%.</p><p><b>CONCLUSION</b>The ITS sequences may serve as reliable molecular markers for identification of Radix Bupleuri.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Spécificité d'espèce
/
Données de séquences moléculaires
/
Chimie
/
Classification
/
Analyse de séquence d'ADN
/
Racines de plante
/
ADN des plantes
/
Espaceur de l'ADN ribosomique
/
Bupleurum
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2006
Type:
Article
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