Temperature-switched high-efficiency D-lactate production from glycerol / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 111-114, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-233264
ABSTRACT
Glycerol from oil hydrolysis industry is being considered as one of the abundent raw materials for fermentation industry. In present study, the aerobic and anaerobic metabolism and growth properties on glycerol by Esherichia coli CICIM B0013-070, a D-lactate over-producing strain constructed previously, at different temperatures were investigated, followed by a novel fermentation process, named temperature-switched process, was established for D-lactate production from glycerol. Under the optimal condition, lactate yield was increased from 64.0% to 82.6%. Subsequently, the yield of D-lactate from glycerol was reached up to 88.9% while a thermo-inducible promoter was used to regulate D-lactate dehydrogenase transcription.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Température
/
Régions promotrices (génétique)
/
Acide lactique
/
Aérobiose
/
Escherichia coli
/
Fermentation
/
Génétique
/
Glycérol
/
Anaérobiose
/
L-Lactate dehydrogenase
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2013
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS