A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer / 南方医科大学学报
Journal of Southern Medical University
;
(12): 627-630, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-233725
ABSTRACT
<p><b>OBJECTIVE</b>To establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer.</p><p><b>METHODS</b>C57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter (24 G). The mice were intravesically pretreated with HCl and then with NaOH, and after washing the bladders with phosphate-buffered saline (PBS), 100 microl (1 x 10(7)) MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C (MMC) administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed.</p><p><b>RESULTS</b>Instillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS.</p><p><b>CONCLUSION</b>We have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Taille d'organe
/
Anatomopathologie
/
Pharmacologie
/
Vessie urinaire
/
Tumeurs de la vessie urinaire
/
Transformation cellulaire néoplasique
/
Mitomycine
/
Lignée cellulaire tumorale
/
Modèles animaux de maladie humaine
/
Souris de lignée C57BL
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2009
Type:
Article
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