Determination of ferulic acid in Fuan oral liquid by high-performance capillary electrophoresis / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To establish a quality control method of Fuan oral liquid.</p><p><b>METHODS</b>High-performance capillary electrophoresis (HPCE) was used to determine the content of feruild acid in Herba taraxaci, and thin-layer chromatography (TLC) was performed to identify Herba taraxaci and Bupleurum chinense. The condition of HPCE was optimized with a fuse silica capillary tube (70 microm x 60 cm) and 20 mmol/L sodium tetraborate buffer (pH9.18) at a constant voltage of 12 kV and temperature at 25 degrees celsius;, with the detection wavelength at 313 nm.</p><p><b>RESULTS</b>Clear spots were displayed on TLC. The calibration curve was linear in the range of 8-40 microg/ml for ferulic acid (Y=360.5-207.4, r=0.9997, n=5). The average recovery rate exceeded 95% with a RSD<3% (n=3).</p><p><b>CONCLUSION</b>This method is simple and specific with a good reproducibility for quality control of Fuan oral liquid.</p>